PYROPHOSPHATE-DEPENDENT 6-PHOSPHOFRUCTOKINASE, A NEW GLYCOLYTIC ENZYME IN PINEAPPLE LEAVES

被引：120

作者：

CARNAL, NW

BLACK, CC

机构：

[1] Biochemistry and Botany Department, University of Georgia, Athens

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 86卷 / 01期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0006-291X(79)90376-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pineapple leaves contain a pyrophosphate-dependent 6-phosphofructokinase which has been partially purified and characterized. In crude extracts the pyrophosphate-dependent activity is 10 to 20-fold higher than the ATP-dependent activity. The partially purified activity is near 2.5 μmol Fru-1,6-P2 formed/min/mg protein. In the reaction 1 Fru-1,6-P2 is formed per 1 pyrophosphate consumed. The enzyme exhibits a pH optimum of 8.0 and the activity is stimulated by Mg++. The discovery of a pyrophosphate-dependent 6-phosphofructokinase in pineapple leaves indicates pyrophosphate can serve as an energy source for synthetic reactions in pineapple and perhaps in other plants as well. © 1979.

引用

页码：20 / 26

页数：7

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