A FLUOROMETRIC METHOD FOR DETERMINING THE DEGREE OF BIOTINYLATION OF PROTEINS

被引：22

作者：

DERBALIAN, GP ^{[1
]}

GOMEZ, B ^{[1
]}

MASINO, RS ^{[1
]}

PARCE, JW ^{[1
]}

机构：

[1] MOLEC DEVICES CORP,4700 BOHANNON DR,MENLO PK,CA 94025

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 126卷 / 02期

关键词：

Avidin; Biotin; Fluorescence; Immunoassay; Streptavidin;

D O I：

10.1016/0022-1759(90)90161-N

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method to determine the number of biotin moieties attached to a protein has been developed based on quenching the natural fluorescence of avidin or streptavidin by biotin. The assay consists of titrating the number of biotin combining sites on streptavidin/avidin before and after adding the biotinylated protein. With this method only those biotin moieties capable of binding to strptavidin.avidin are detected. The assay is simple and sensitive, requiring only 1-10 μg of biotinylated protein per determination. © 1990.

引用

页码：281 / 285

页数：5