THE EFFECTS OF CRYOPRESERVATION AND THAWING ON THE DEVELOPMENT INVITRO AND INVIVO OF BIOPSIED 8-CELL MOUSE EMBRYOS

被引：22

作者：

KRZYMINSKA, U ^{[1
]}

ONEILL, C ^{[1
]}

机构：

[1] ROYAL N SHORE HOSP,HUMAN REPROD UNIT,ST LEONARDS,NSW 2065,AUSTRALIA

来源：

HUMAN REPRODUCTION | 1991年 / 6卷 / 06期

关键词：

BIOPSY; CRYOPRESERVATION; EMBRYO; MICROMANIPULATION; PRENATAL DIAGNOSIS;

D O I：

10.1093/oxfordjournals.humrep.a137437

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The possible impact of cryopreservation on biopsied 8-cell mouse embryos was investigated. Biopsied and control 8-cell embryos were cryopreserved using a slow freezing and quick thawing protocol with 1,2-propanediol as a cryoprotectant. The cryopreservation process did not affect either the recovery or the survival of biopsied embryos, when compared with intact controls; however, sham controls survived significantly better than biopsied 8-cell embryos (88.6 versus 74.2%, P < 0.001). When fully and partially intact surviving embryos were cultured in vitro to the blastocyst stage, there was no difference in the proportions of embryos which formed blastocysts (biopsy 97.2%, intact control 98.4% and sham control 93.7%). The developmental potential and fetal development in vivo following embryo transfer were not impaired when assessed on day 17 of pregnancy. Cryopreservation of biopsied 8-cell mouse embryos is therefore a feasible approach to storing embryos while analysis of the biopsied material is carried out.

引用

页码：832 / 835

页数：4

共 20 条

[1]

Ashwood-Smith M.J., Morris G.W., Fowler R., Appicton T.C., Ashom R., Physical factors are involved in the destruction of embryos and oocytes during freezing and thawing procédures, Hum. Reprod, 3, pp. 795-802, (1988)

[2]

Cohen J., Simons R.S., Fehilly C.B., Edwards R.G., Factors affecting survival and implantation of cryopreserved human embryos. J, Vitro Fertii. Embryo Transfer, 3, pp. 46-52, (1986)

[3]

Depypere H., Carroll J., Mathews C.D., Vanderkerckhove D., Comhaire F., Freezing and thawing of embryos previously undergoing zona drilling, Hum. Reprod, 3, (1988)

[4]

Garrisi G.J., Talansky B.E., Navot D., Gordon J., Successful Cryopreservation of Zona-Drilled Mouse Embryos. Sixth World Congress ¡VFand Alternóte Assisted Reproduction, (1989)

[5]

Gordon J.W., Gang J., Use of zona drilling for safe and effective biopsy of mutine oocytes and embryos, Bioi. Reprod, 42, pp. 869-879, (1990)

[6]

Handyside A.H., Kantongianni E.H., Hardy K., Winston R.M., Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification, Nature, 344, pp. 768-770, (1990)

[7]

Holding C., Monk M., Diagnosis of /3-thalassaemia by DNA amplification in single blastomeres from mouse pre-implantation embryos, Lancet, 3, pp. 532-535, (1989)

[8]

Krzyminska U., Lutjen J., O'neill C., Assessment of the viability and pregnancy potential of mouse embryos biopsied at different preimplantation stages of development, Hum. Reprod, 5, pp. 203-208, (1990)

[9]

Lassalle B., Testart J., Renard J.P., Human embryo features that influence the success of cryopreservation with the use of 1, 2- propanediol, Fertii. Steril, 44, pp. 645-651, (1985)

[10]

Lindeman R., Lutjen J., O'neill C., Trent R.J., Exclusion of ß-thalassaemia by biopsy and DNA amplification in mouse pre-embryos, Prenat. Diagn, 10, pp. 295-301, (1990)

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