ANALYSIS OF GLYCOFORMS PRESENT IN 2 MOUSE IGG2A MONOCLONAL-ANTIBODY PREPARATIONS

被引：13

作者：

COCOMARTIN, JM ^{[1
]}

BRUNINK, F ^{[1
]}

VANDERVELDENDEGROOT, TAM ^{[1
]}

BEUVERY, EC ^{[1
]}

机构：

[1] NATL INST PUBL HLTH & ENVIRONM PROTECT,3720 BA BILTHOVEN,NETHERLANDS

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1992年 / 155卷 / 02期

关键词：

MONOCLONAL ANTIBODY; CARBOHYDRATE HETEROGENEITY; LECTIN;

D O I：

10.1016/0022-1759(92)90291-Z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Lectins have been used for the determination of the oligosaccharide structures expressed by two monoclonal IgG antibodies, MN12 and RIV6. Dot blot experiments revealed the presence of terminal Fucalpha(1 --> 6)GlcNAc, Galbeta(1 --> 3)GalNAc, Galbeta(1 --> 4)GlcNAc, Manalpha(1 --> 6, 1 --> 3)Man, NeuAcalpha(2 --> 6)Gal and NeuAcalpha(2 --> 6)GalNAc on both monoclonal antibodies. MN12 was shown to contain a carbohydrate moiety within the Fc region only. RIV6 contained carbohydrate moieties within both the Fc and Fab regions. Additional O-glycosidic linked carbohydrate chains were detected within the Fc region of both monoclonal antibodies. High mannose structures were also detected on both Mabs.

引用

页码：241 / 248

页数：8

共 36 条

[1] BAENZIGER J, 1974, J BIOL CHEM, V249, P7270
[2] GLYCOSYLATION OF IGG, IMMUNE-COMPLEXES AND IGG SUBCLASSES IN THE MRL-LPR/LPR MOUSE MODEL OF RHEUMATOID-ARTHRITIS
BOND, A
COOKE, A
HAY, FC
[J]. EUROPEAN JOURNAL OF IMMUNOLOGY, 1990, 20 (10) : 2229 - 2233
[3] BOREL IM, 1990, IMMUNOLOGY, V70, P281
[4] IMMUNOGLOBULIN-G - FUNCTIONAL SITES
BURTON, DR
[J]. MOLECULAR IMMUNOLOGY, 1985, 22 (03) : 161 - 206
[5] O-LINKED OLIGOSACCHARIDES FROM HUMAN-SERUM IMMUNOGLOBULIN-A1
FIELD, MC
DWEK, RA
EDGE, CJ
RADEMACHER, TW
[J]. BIOCHEMICAL SOCIETY TRANSACTIONS, 1989, 17 (06) : 1034 - 1035
[6] FUJII S, 1990, J BIOL CHEM, V265, P6009
[7] Goding JW, 1986, MONOCLONAL ANTIBODIE
[8] ENVIRONMENTAL-EFFECTS ON PROTEIN GLYCOSYLATION
GOOCHEE, CF
MONICA, T
[J]. BIO-TECHNOLOGY, 1990, 8 (05): : 421 - 427
[9] QUALITY-CONTROL OF MURINE MONOCLONAL-ANTIBODIES USING ISOELECTRIC-FOCUSING AFFINITY IMMUNOBLOT ANALYSIS
HAMILTON, RG
REIMER, CB
RODKEY, LS
[J]. HYBRIDOMA, 1987, 6 (02): : 205 - 217
[10] STRUCTURAL CHARACTERIZATION OF GLYCOPROTEIN CARBOHYDRATE CHAINS BY USING DIGOXIGENIN-LABELED LECTINS ON BLOTS
HASELBECK, A
SCHICKANEDER, E
VONDERELTZ, H
HOSEL, W
[J]. ANALYTICAL BIOCHEMISTRY, 1990, 191 (01) : 25 - 30

← 1 2 3 4 →