IMMUNOPHOTODIAGNOSIS OF COLON CARCINOMAS IN PATIENTS INJECTED WITH FLUORESCEINATED CHIMERIC ANTIBODIES AGAINST CARCINOEMBRYONIC ANTIGEN

被引:83
作者
FOLLI, S
WAGNIERES, G
PELEGRIN, A
CALMES, JM
BRAICHOTTE, D
BUCHEGGER, F
CHALANDON, Y
HARDMAN, N
HEUSSER, C
GIVEL, JC
CHAPUIS, G
CHATELAIN, A
VANDENBERGH, H
MACH, JP
机构
[1] ECOLE POLYTECH FED LAUSANNE,INST EXPTL PHYS,CH-1015 LAUSANNE,SWITZERLAND
[2] ECOLE POLYTECH FED LAUSANNE,INST ENVIRONM ENGN,CH-1015 LAUSANNE,SWITZERLAND
[3] CHU VAUDOIS,DEPT SURG,CH-1011 LAUSANNE,SWITZERLAND
[4] CIBA GEIGY AG,CH-4002 BASEL,SWITZERLAND
关键词
D O I
10.1073/pnas.89.17.7973
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Based on previous experiments in nude mice, showing that fluoresceinated monoclonal antibodies against carcinoembryonic antigen localized specifically in human carcinoma xenografts and could be detected by laser-induced fluorescence, we performed a feasibility study to determine whether this immunophotodiagnosis method could be applied in the clinic. Six patients, with known primary colorectal carcinoma, received an i.v. injection of 4.5 or 9 mg of mouse-human chimeric anti-carcinoembryonic antigen monoclonal antibody coupled with 0.10-0.28 mg of fluorescein (molar ratio 1/10 to 1/14). The monoclonal antibody was also labeled with 0.2-0.4 mCi of I-125 (1 Ci = 37 GBq). Photodetection of the tumor was done ex vivo on surgically resected tissues for the six patients and in vivo by fluorescence rectosigmoidoscopy for the sixth patient. Upon laser irradiation, clearly detectable heterogeneous green fluorescence from the dye-antibody conjugate was visually observed on all six tumors; almost no such fluorescence was detectable on normal mucosa. The yellowish tissue autofluorescence, which was emitted from both tumor and normal mucosa, could be subtracted by real-time image processing. Radioactivity measurements confirmed the specificity of tumor localization by the conjugate; tissue concentrations of up to 0.059% injected dose per g of tumor and 10 times less (0.006%) per g of normal mucosa were found. The overall results demonstrate the feasibility of tumor immunophotodiagnosis at the clinical level.
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页码:7973 / 7977
页数:5
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