6,7-DIMETHOXY-1-METHYL-2-OXO-1,2-DIHYDROQUINOXALIN-3-YLPROPIONOHYDRAZIDE AS A FLUORESCENCE DERIVATIZATION REAGENT FOR ALDEHYDES IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引:13
作者
IWATA, T [1 ]
HIROSE, T [1 ]
NAKAMURA, M [1 ]
YAMAGUCHI, M [1 ]
机构
[1] FUKUOKA UNIV,FAC PHARMACEUT SCI,JONAN KU,FUKUOKA 81401,JAPAN
关键词
6,7-DIMETHOXY-1-METHYL-2-OXO-1,2-DIHYDROQUINOXALIN-3-YLPROPIONOHYDRAZIDE; ALDEHYDE; FLUORESCENCE DERIVATIZATION REAGENT; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY;
D O I
10.1039/an9931800517
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
6,7-Dimethoxy-1-methyl-2-oxo-1,2-dihydroquinoxalin-3-ylpropionohydrazide has been developed as a highly sensitive and selective fluorescence derivatization reagent for aliphatic and aromatic aldehydes. The reaction conditions were optimized with cyclohexanecarbaldehyde, 2-naphthaldehyde and heptanal. The reagent reacted with the aldehydes in the presence of trichloroacetic acid at 60-degrees-C to produce the corresponding fluorescent derivatives, which were separated on a reversed-phase column, L-column ODS, by isocratic elution with 70% v/v aqueous methanol as eluent. The derivatives were detected spectrofluorimetrically at 442 nm with excitation at 362 nm. The detection limits (signal-to-noise ratio = 3) for the aldehydes are 13-18 fmol for an injection volume of 10 mul.
引用
收藏
页码:517 / 519
页数:3
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