IN-VIVO LABELING OF NICOTINIC CHOLINERGIC RECEPTORS IN BRAIN WITH [H-3] CYTISINE

被引：34

作者：

FLESHER, JE

SCHEFFEL, U

LONDON, ED

FROST, JJ

机构：

[1] JOHNS HOPKINS MED INST, DEPT RADIOL, BALTIMORE, MD 21287 USA

[2] NIDA, ADDICT RES CTR, BALTIMORE, MD 21224 USA

[3] UNIV MARYLAND, SCH MED, BALTIMORE, MD 21201 USA

来源：

LIFE SCIENCES | 1994年 / 54卷 / 24期

关键词：

H-3] CYTISINE; CENTRAL NICOTINIC CHOLINERGIC RECEPTORS; MOUSE BRAIN; IN VIVO BINDING;

D O I：

10.1016/0024-3205(94)90146-5

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

[H-3]Cytisine was evaluated as an in vivo ligand for the nicotinic cholinergic receptor (nAchR) in mouse brain. The tracer was injected intravenously, and radioactivity in brain regions was analyzed. Radioactivity peaked in the brain at 30 minutes. It was highest in the thalamus, intermediate in the superior colliculi, prefrontal cortex and hippocampus, and low in the cerebellum. Pretreatment with unlabeled cytisine inhibited binding in the thalamus, but not in the cerebellum. Binding was displaced by 1-nicotine, but not by d-nicotine or dexetimide. The results suggest that cytisine, appropriately labeled with a positron emitting radionuclide, may be useful for study of nicotinic cholinergic receptors in humans by emission computed tomography.

引用

页码：1883 / 1890

页数：8

共 24 条

[1] INVIVO SPECIFIC BINDING OF [H-3] 1-NICOTINE IN THE MOUSE-BRAIN [J].