TOWARDS AN UNDERSTANDING OF THE MOLECULAR-BASIS OF PLANTS K+ TRANSPORT - CHARACTERIZATION OF CLONED K+ TRANSPORT CDNAS

被引：24

作者：

KOCHIAN, LV ^{[1
]}

GARVIN, DF ^{[1
]}

SHAFF, JE ^{[1
]}

CHILCOTT, TC ^{[1
]}

LUCAS, WJ ^{[1
]}

机构：

[1] UNIV CALIF DAVIS, PLANT BIOL SECT, DAVIS, CA 95616 USA

来源：

PLANT AND SOIL | 1993年 / 155卷

关键词：

CDNA; CLONING; HETEROLOGOUS EXPRESSION; HIGH-AFFINITY K+ TRANSPORT; K+ CHANNEL; K+ TRANSPORT; OOCYTE; ROOT; YEAST;

D O I：

10.1007/BF00024997

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Recently, two K+-transport cDNAs, KAT1 and AKT1, were cloned in Arabidopsis thaliana. These cDNAs had structural similarities to K+ channel genes in animals, and also conferred the ability for growth on micromolar levels of K+ when expressed in K+ transport-defective yeast mutants. In this study, we examined the possibility that KAT1 encodes the high-affinity K+ transport system that has been previously characterized in plant roots, by studying the concentration-dependent kinetics of K+ transport for KAT1 expressed in Xenopus oocytes and Saccharomyces cerevisiae. In both organisms, the K+ transport system encoded by KAT1 yielded Michaelis-Menten kinetics with a high K-m for K+ (35 mM in oocytes, 0.6 mM in yeast cells). Furthermore, Northern analysis indicated that KAT1 is expressed primarily in the Arabidopsis shoot. These results strongly suggest that the system encoded by KAT1 is not a root high-affinity K+ transporter.

引用

页码：115 / 118

页数：4

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