TRYPANOSOMA-CRUZI - SHEDDING OF SURFACE-ANTIGENS AS MEMBRANE-VESICLES

被引：138

作者：

GONCALVES, MF

UMEZAWA, ES

KATZIN, AM

DESOUZA, W

ALVES, MJM

ZINGALES, B

COLLI, W

机构：

[1] UNIV SAO PAULO, FAC MED, INST MED TROP, SAO PAULO, BRAZIL

[2] UNIV SAO PAULO, INST CIENCIAS BIOMED, DEPT PARASITOL, BR-05508 SAO PAULO, BRAZIL

[3] UNIV FED RIO DE JANEIRO, INST BIOFIS CARLOS CHAGAS FILHO, BR-21941 RIO DE JANEIRO, BRAZIL

来源：

EXPERIMENTAL PARASITOLOGY | 1991年 / 72卷 / 01期

关键词：

TRYPANOSOMA-CRUZI; PARASITE HEMOFLAGELLATE; SHEDDING; SURFACE ANTIGENS; VESICLES; BOVINE SERUM ALBUMIN (BSA); DULBECCOS MODIFIED EAGLE MEDIUM (DMEM); FETAL CALF SERUM (FCS); HYPERIMMUNE SERUM (HIS); POLYACRYLAMIDE GEL ELECTROPHORESIS (PAGE); SODIUM DODECYL SULFATE (SDS); TRICHLOROACETIC ACID (TCA); TISSUE CULTURE TRYPOMASTIGOTES (TCT);

D O I：

10.1016/0014-4894(91)90119-H

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Tissue culture-derived trypomastigotes from Trypanosoma cruzi spontaneously shed surface antigens into the culture medium. The shedding is a temperature-and time-dependent phenomenon and is independent of the presence of proteins or immune serum in the medium. The analysis of this process in four strains (Y, YuYu, CA1, and RA) showed differences in the amounts of polypeptides released. However, for all strains the liberation of the entire set of surface polypeptides ranging in molecular mass from 70 to 150 kDa was observed. Biochemical and electron microscopic data strongly suggest that most of the surface antigens are released as plasma membrane vesicles, ranging from 20 to 80 nm in diameter. © 1991.

引用

页码：43 / 53

页数：11

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