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NEW PATHOGENICITY LOCI IN ERWINIA-STEWARTII IDENTIFIED BY RANDOM TN5 MUTAGENESIS AND MOLECULAR-CLONING
被引：13
作者：
COPLIN, DL
FREDERICK, RD
MAJERCZAK, DR
机构：
关键词：
STEWART WILT;
ZEA-MAYS;
D O I：
10.1094/MPMI-5-266
中图分类号：
Q5 [生物化学];
Q7 [分子生物学];
学科分类号：
071010 ;
081704 ;
摘要：
Pseudomonas aeruginosa plasmid pMO75 was used as a delivery system to introduce random Tn5 mutations into the genome of Erwinia stewartii. Nineteen avirulent mutants were identified by screening for loss of wilt induction and water-soaking (Wts) on corn seedlings. Five new wts mutations were mapped to the left of wtsA, thereby enlarging the wts gene cluster to approximately 28 kb. Four Wts- mutations appeared to be unlinked to the wts cluster and may represent new pathogenicity loci.
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页码：266 / 268
页数：3
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