DNA DEAMINATING ABILITY AND GENOTOXICITY OF NITRIC-OXIDE AND ITS PROGENITORS

被引：1119

作者：

WINK, DA

KASPRZAK, KS

MARAGOS, CM

ELESPURU, RK

MISRA, M

DUNAMS, TM

CEBULA, TA

KOCH, WH

ANDREWS, AW

ALLEN, JS

KEEFER, LK

机构：

[1] NCI,FREDERICK CANC RES & DEV CTR,COMPARAT CARCINOGENESIS NATL LAB,FREDERICK,MD 21701

[2] US FDA,ROCKVILLE,MD 20852

[3] GLAXO INC,RES TRIANGLE PK,NC 27709

[4] US FDA,WASHINGTON,DC 20204

[5] NCI,FREDERICK CANC RES & DEV CTR,PRI DYNCORP,FREDERICK,MD 21701

来源：

SCIENCE | 1991年 / 254卷 / 5034期

关键词：

D O I：

10.1126/science.1948068

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nitric oxide (NO), a multifaceted bioregulatory agent and an environmental pollutant, can also cause genomic alterations. In vitro, NO deaminated deoxynucleosides, deoxynucleotides, and intact DNA at physiological pH. That similar DNA damage can also occur in vivo was tested by treating Salmonella typhimurium strain TA1535 with three NO-releasing compounds, including nitroglycerin. All proved mutagenic. Observed DNA sequence changes were > 99% C --> T transitions in the hisG46 (CCC) target codon, consistent with a cytosine-deamination mechanism. Because exposure to endogenously and exogenously produced NO is extensive, this mechanism may contribute to the incidence of deamination-related genetic disease and cancer.

引用

页码：1001 / 1003

页数：3

共 47 条

[1] LOCALIZATION OF NITRIC-OXIDE SYNTHASE INDICATING A NEURAL ROLE FOR NITRIC-OXIDE [J].