THE DETECTION OF SALMONELLA-ENTERITIDIS AND SALMONELLA-TYPHIMURIUM USING IMMUNOMAGNETIC SEPARATION AND CONDUCTANCE MICROBIOLOGY

A rapid method for detection of Salmonella in milk powder is described. The technique involves immunomagnetic separation of Salmonella from pre-enrichment broths using new commercially-available materials, and detection using conductance measurements. Salmonella detection was enhanced by reducing the number and types of competing bacteria present and concentrating the number of Salmonella in the final assay. After a 6 h pre-incubation period Salmonella enteritidis, from an initial inoculum size of 20 cells/ml, were detected in 7-5 h by conductance.