PHOSPHORYLATION IS INVOLVED IN TRANSCRIPTIONAL ACTIVATION BY THE 1,25-DIHYDROXYVITAMIN-D3 RECEPTOR

被引：39

作者：

DARWISH, HM ^{[1
]}

BURMESTER, JK ^{[1
]}

MOSS, VE ^{[1
]}

DELUCA, HF ^{[1
]}

机构：

[1] UNIV WISCONSIN,COLL AGR & LIFE SCI,DEPT BIOCHEM,420 HENRY MALL,MADISON,WI 53706

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1167卷 / 01期

关键词：

VITAMIN-D; CALCIUM; BONE CALCIFICATION; STEROID; RECEPTOR;

D O I：

10.1016/0005-2760(93)90213-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The 1,25-dihydroxyvitamin D3 receptor becomes phosphorylated upon treatment with 1,25-dihydroxyvitamin D3. We have investigated the role of phosphorylation in the transcriptional activity induced by 1,25-dihydroxyvitamin D3 through its receptor. An active 1,25-dihydroxyvitamin D3-dependent transcription system was reconstituted in CV-1 cells by co-transfection of plasmids containing the rat 1,25-(OH)2D3 receptor DNA and a functional vitamin D response element (DRE) in a reporter gene construct. Treatment of these transiently transfected CV-1 cells with modulators of protein kinase A (8-Br-cAMP, PKIA and H-9) and phosphatases (Okadaic acid) resulted in mimicking or abolishing the transcriptional activity of 1,25-dihydroxyvitamin D3 in a receptor-dependent fashion. These modulators directly altered 1,25-dihydroxyvitamin D3 receptor phosphorylation. Therefore, the present results strongly suggest that phosphorylation plays a central role in the transcriptional activity of the 1,25-dihydroxyvitamin D3 receptor.

引用

页码：29 / 36

页数：8

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