DISTRIBUTION AND MICROCHEMICAL DETECTION OF PHENOLIC-ACIDS, FLAVONOIDS, AND PHENOLIC-ACID AMIDES IN MAIZE KERNELS

被引：53

作者：

SEN, A

BERGVINSON, D

MILLER, SS

ATKINSON, J

FULCHER, RG

ARNASON, JT

机构：

[1] UNIV OTTAWA,DEPT BIOL,OTTAWA K1N 6N5,ON,CANADA

[2] AGR CANADA,RES BRANCH,OTTAWA K1A 0C6,ON,CANADA

[3] BROCK UNIV,DEPT CHEM,ST CATHARINES L2S 3A1,ON,CANADA

[4] UNIV MINNESOTA,DEPT FOOD SCI & NUTR,ST PAUL,MN 55108

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1994年 / 42卷 / 09期

关键词：

ZEA MAYS; FERULIC ACID; FLAVONOIDS; AMINES; FLUORESCENCE; MICROSPECTROFLUOROMETRY;

D O I：

10.1021/jf00045a009

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Because of the importance of phenolics in the resistance of maize kernels to pests, microspectrofluorometry was employed as a new method of mapping the location of these substances in maize kernels. Autofluorescence due to phenolic acids was detected mainly in the embryo, aleurone, and pericarp of maize kernel cross sections. Resistant maize types showed higher intensities of phenolic fluorescence but no unusual distributions. Borinic acid reagent enhanced fluorescence due to flavonoids in the aleurone layer, and Ehrlich's reagent revealed phenolic acid amide fluorescence in the embryo and aleurone. The localization of phenolic amines was confirmed by HPLC analysis.

引用

页码：1879 / 1883

页数：5

共 26 条

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