RESPONSE OF GUINEA-PIG SMOOTH AND STRIATED URETHRAL SPHINCTER TO CROMAKALIM, PRAZOSIN, NIFEDIPINE, NITROPRUSSIDE, AND ELECTRICAL-STIMULATION

被引：15

作者：

VONHEYDEN, B ^{[1
]}

RIEMER, RK ^{[1
]}

NUNES, L ^{[1
]}

BROCK, GB ^{[1
]}

LUE, TF ^{[1
]}

TANAGHO, EA ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO,SCH MED,DEPT UROL,SAN FRANCISCO,CA 94143

来源：

NEUROUROLOGY AND URODYNAMICS | 1995年 / 14卷 / 02期

关键词：

STRIATED URETHRAL SPHINCTER; GUINEA PIG; DECAMETHONIUM; HEXAMETHONIUM; PRAZOSIN; CROMAKALIM; NIFEDIPINE; NITRIC OXIDE; VIP;

D O I：

10.1002/nau.1930140208

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Prazosin (an alpha-l-adrenegic blocker) and cromakalim (potassium channel opener), given alone, induced significant fatigue of the urethral sphincter at a concentration of 10(-4) M; both drugs combined achieved a significant sphincteric fatigue at a concentration of 10(-5) M each. To 10(-4) M hexamethonium (ganglionic smooth muscle blocker) and 10(-4) M decamethonium (nicotinic blocker of striated muscle) the striated urethral sphincter responded like striated muscle with no detectable function of its smooth muscle component. Therefore, the striated component seems to play a dominant role in sphincteric function. With calcium depletion or in the presence of a calcium channel blocker (10(-4) M nifedipine) the urethral sphincter showed a relative enhancement of response to electrical field stimulation when compared with smooth and skeletal muscle, whose responses were both significantly reduced. This phenomenon could not be explained with calcium-dependent, inhibitory, nitric oxide-releasing nerves, as the NO-synthase blocker N-nitro-L-arginine (10(-5) M to 5 x 10(-5) M) failed to induce the enhancement of sphincter contraction during electrostimulation found with calcium depletion. Still, NO-releasing nerves might play a role in sphincteric relaxation because sodium nitroprusside (10(-5) M) induced a significant relaxation of the urethral sphincter precontracted with 80 mM potassium. The potential to weaken sphincteric closure with drugs, exemplified by the results obtained in response to prazosin and cromakalim, would represent a therapeutic advance in the patient with neurogenic bladder dysfunction. (C) 1995 Wiley-Liss. Inc.

引用

页码：153 / 168

页数：16

共 38 条

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