ONLINE ANALYSIS OF AVERMECTIN FERMENTATION CELL-GROWTH KINETICS IN AN INDUSTRIAL PILOT-PLANT

被引：14

作者：

GBEWONYO, K

JAIN, D

HUNT, G

DREW, SW

BUCKLAND, BC

机构：

[1] Department of Biochemical Engineering, Merck Sharp and Dohme Research Laboratories, Rahway, NJ 07065, United States

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1989年 / 34卷 / 02期

关键词：

Cell Culture--Growth Kinetics - Chemical Reactions--Fermentation - Computers--Applications - Mass Spectrometers - Oxygen;

D O I：

10.1002/bit.260340212

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Experimental data are presented which show that on-line calculation of oxygen uptake rate can be used to estimate the cell concentration of Streptomyces avermitilis during the active growth phase of this fermentation. Moreover, by dividing the oxygen uptake rate by the total oxygen consumed, an on-line estimate of specific growth rate of this culture can be generated. A theoretical basis is provided for this model. Use of a mass spectrometer for vent gas analysis coupled with computer data acquisition has made this information both very accurate and readily available. Examples are given which illustrate the kinetics of the avermectin fermentation as well as the effect of a temperature shift on the specific growth rate.

引用

页码：234 / 241

页数：8

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