AN IMPROVED METHOD FOR THE DETECTION OF DCM METHYLATION IN DNA-MOLECULES

被引：13

作者：

REUTER, M

PEIN, CD

BUTKUS, V

KRUGER, DH

机构：

[1] HUMBOLDT UNIV,BEREICH MED CHARITE,INST VIROL,SCHUMANNSTR 20-21,O-1040 BERLIN,GERMANY

[2] HUMBOLDT UNIV,DEPT CHEM,O-1040 BERLIN,GERMANY

[3] VILNIUS APPL ENZYMOL INST,VILNIUS 232028,LITHUANIA,USSR

来源：

GENE | 1990年 / 95卷 / 01期

关键词：

bacteriophage M13; DNA methylation; oligodeoxyribonucleotide duplexes; restriction endonuclease BstNI; restriction endonuclease EcoRII; restriction endonuclease MvaI; vector M13mp18;

D O I：

10.1016/0378-1119(90)90429-U

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The intrinsic insensitivity of EcoRII recognition sites in RF DNAs of phage M13 and vector M13mp18 towards this restriction endonuclease can be overcome by adding site-specific oligodeoxyribonucleotide duplexes to the restriction sample. Since Dcm- DNA but not Dcm+-methylated DNA becomes susceptible under these conditions, this procedure constitutes an improvement of the Dcm methylation assay. © 1990.

引用

页码：161 / 162

页数：2

共 8 条

[1] EXPRESSION OF THE CLONED COLIPHAGE T3 S-ADENOSYLMETHIONINE HYDROLASE GENE INHIBITS DNA METHYLATION AND POLYAMINE BIOSYNTHESIS IN ESCHERICHIA-COLI [J].