NO RELATIONSHIP BETWEEN THE AGE-RELATED DECREASE IN PROSTACYCLIN PRODUCTION AND THE LEVEL OF INTRACELLULAR LIPID-PEROXIDATION IN HUMAN UMBILICAL VEIN ENDOTHELIAL-CELLS IN CULTURE

Some investigators have speculated that a decrease in prostacyclin production observed during ageing of endothelial cells is caused by an increase in intracellular lipid peroxide. We checked this speculation using an in vitro model to study the ageing of human vascular endothelial cells. For this purpose we determined the levels of intracellular lipid peroxide of endothelial cells at various culture ages, and found that the level of intracellular lipid peroxidation did not increase during in vitro ageing. Amounts of intracellular lipid peroxide also differed depending on the growth phase, the addition of heparin and the strain of endothelial cells, but the cells producing prostacyclin at a low level did not necessarily contain larger amounts of intracellular lipid peroxide. Therefore, we postulate that the age-related decrease in prostacyclin production is not due to an increase in the amount of intracellular lipid peroxide as a function of age.