PREDICTION OF SINGLE AND BINARY PROFILES IN OVERLOADED ELUTION CHROMATOGRAPHY USING VARIOUS SEMI-IDEAL MODELS

Experimental band profiles of 2-phenylethanol and 3-phenylpropanol were recorded for pure components in different amounts and for binary mixtures at different compositions in reversed-phase chromatography. The injection function was also measured. These experimental profiles are compared with those calculated using different finite difference methods (i.e., the Craig and Rouchon et al. models). The results show that it is important to take into account the true injection profile. The different calculation procedures in most instances give profiles which are in close agreement with the experimental data. The Craig model gives profiles which are generally sharper than those given by the Rouchon et al. model. Differences between the experimental and calculated profiles are ascribed to mathematical properties of the method implemented.