A SIMPLE AND MULTIPURPOSE CONCENTRATION-CLAMP METHOD FOR RAPID SUPERFUSION

A simple method for the rapid exchange of the medium used for a variety of electrophysiological experiments including patch-clamp experiments on cultured cells is described. The advantages of this method over the conventional ones are that this solution exchange system has a very simple structure and thus is inexpensive and can be easily installed in already existing experimental setups. The method involves the use of a fine double-barrelled polyethylene needle placed near the cell or other isolated preparations under recording. Electromagnetic valves are used to switch the solutions in the polyethylene tubes which enable a rapid and localized application of test solutions as well as their rapid washout. The latency of solution exchange, as measured by a change in the holding current under voltage-clamp experiments was fairly rapid (about 120 ms). Neither was the recording interrupted nor the sealing resistance affected during repeated exposure to test solutions. This method is proving to be useful, particularly for electrophysiological and pharmacological studies on immovable cells such as those in culture.