AN EXON-TRAPPING SYSTEM WITH A NEWLY CONSTRUCTED TRAPPING VECTOR PEXT2 - ITS APPLICATION TO THE PROXIMAL REGION OF THE HUMAN CHROMOSOME-21 LONG ARM

被引：7

作者：

OZAWA, N

KANO, T

TAGA, C

HATTORI, M

SAKAKI, Y

SUZUKI, H

机构：

[1] SHIONOGI INST MED SCI,2-5-1 MISHIMA,SETTSU CITY,OSAKA 566,JAPAN

[2] UNIV TOKYO,INST MED SCI,MINATO KU,TOKYO 108,JAPAN

来源：

FEBS LETTERS | 1993年 / 325卷 / 03期

关键词：

GENE CLONING; PLASMID VECTOR; RNA SPLICING; CHROMOSOME-21; NEUROFIBROMATOSIS-1; ALZHEIMERS DISEASE;

D O I：

10.1016/0014-5793(93)81094-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed an exon-trapping system with a newly constructed trapping vector containing multiple cloning sites (designated pEXT2). The system revealed high sensitivity for trapping a control exon from several hundred kbp of DNA. We have applied the system to the cosmid clones located on human chromosome 21p11-q21, and identified two fragments highly homologous to neurofibromatosis 1 (NF1) gene and a clearly transcribed fragment hybridized with approximately 1.6 kb RNA from human brain and human glioblastoma A172 cell.

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页码：303 / 308

页数：6

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