CRYOPRESERVATION OF SPORES OF CYATHEA-SPINULOSA WALL EX HOOK F - AN ENDANGERED TREE FERN

被引：24

作者：

AGRAWAL, DC ^{[1
]}

PAWAR, SS ^{[1
]}

MASCARENHAS, AF ^{[1
]}

机构：

[1] NATL CHEM LAB,DIV PLANT TISSUE CULTURE,POONA 411008,MAHARASHTRA,INDIA

来源：

JOURNAL OF PLANT PHYSIOLOGY | 1993年 / 142卷 / 01期

关键词：

CYATHEA-SPINULOSA; CRYOPRESERVATION; ENDANGERED PLANT; TREE FERN;

D O I：

10.1016/S0176-1617(11)80121-5

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Spores of Cyathea spinulosa, an endangered tree fern, survived after storage in liquid nitrogen (- 196-degrees-C). The maximum germination of spores (93.5 %) after retrieval from liquid nitrogen was obtained upon slow thawing at 30-degrees-C and culturing of these spores on a modified Knudson's medium without sucrose. Culturing on Murashige and Skoog's medium, incorporation of sucrose and rapid thawing methods resulted in a comparatively lower percentage of spore germination.

引用

页码：124 / 126

页数：3

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