LIPID-PEROXIDATION CONTRIBUTES TO CARDIAC DEFICITS AFTER ISCHEMIA AND REPERFUSION OF THE SMALL-BOWEL

被引:25
作者
HORTON, JW
WHITE, DJ
机构
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1993年 / 264卷 / 05期
关键词
MESENTERIC ISCHEMIA; MALONDIALDEHYDE; CARDIAC CONTRACTILITY; LANGENDORFF PREPARATION;
D O I
10.1152/ajpheart.1993.264.5.H1686
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Our previous studies showed that intestinal ischemia-reperfusion (IR) impairs cardiac contractile function. The present study examined the contribution of oxygen free radicals and lipid peroxidation of cardiac cell membrane to cardiac dysfunction after intestinal IR in a rat model of superior mesenteric artery (SMA) occlusion (atraumatic clip for 20 min) and collateral arcade ligation. Controls were sham operated (group 1, n = 25). In group 2, 30 rats with SMA occlusion were killed 3-4 h after reperfusion without treatment. Amino-steroid (U-74389F), a pharmacological agent known to inhibit lipid peroxidation of membranes, was given 1 min before occlusion of the SMA (group 3, n = 19). All rats were killed 3-4 h after reperfusion of the ischemic intestine, and the hearts were harvested for in vitro assessment of cardiac function (Langendorff preparation). Cardiac contractile depression occurred in the untreated group as indicated by a fall in left ventricular pressure (from 76 +/- 3 to 64 +/- 3 mmHg, P = 0.01), maximum +dP/dt (from 1,830 +/- 60 to 1,577 +/- 64 mmHg/s, P = 0.05), and maximum -dP/dt (from 1,260 +/- 50 to 950 +/- 60 mmHg/s, P = 0.005). Lipid peroxidation of cardiac membranes occurred after untreated IR as indicated by the rise in cardiac malondialdehyde levels (MDA) (from 0.203 +/- 0.046 to 0.501 +/- 0.044 nM/mg protein, P < 0.05). Aminosteroid administration at the time of SMA occlusion prevented IR-mediated cardiac dysfunction as indicated by left ventricular pressure (88 +/- 3 mmHg), +dP/dt (2,040 +/- 89 mmHg/s), and -dP/dt (1,721 +/- 82 mmHg/s) values equivalent to those measured in control hearts; in addition, left ventricular function curves for aminosteroid-treated rats were identical to those generated by control hearts. Myocardial MDA levels fell to 0.123 +/- 0.012 nM/mg protein 3 h after aminosteroid-treated IR. We concluded that oxygen-derived free radicals produced during intestinal ischemia and reperfusion promote lipid peroxidation of cardiac cell membranes, contributing to cardiac dysfunction.
引用
收藏
页码:H1686 / H1692
页数:7
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