IRON REGULATORY PROTEIN PREVENTS BINDING OF THE 43S TRANSLATION PRE-INITIATION COMPLEX TO FERRITIN AND EALAS MESSENGER-RNAS

被引：226

作者：

GRAY, NK ^{[1
]}

HENTZE, MW ^{[1
]}

机构：

[1] EUROPEAN MOLEC BIOL LAB,GENE EXPRESS PROGRAMME,D-69117 HEIDELBERG,GERMANY

来源：

EMBO JOURNAL | 1994年 / 13卷 / 16期

关键词：

EALAS; IRON-RESPONSIVE ELEMENTS; POSTTRANSCRIPTIONAL REGULATION; RNA-PROTEIN INTERACTIONS; RIBOSOME BINDING;

D O I：

10.1002/j.1460-2075.1994.tb06699.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Translation of ferritin and erythroid 5-aminolevulinate synthase (eALAS) mRNAs is regulated by iron via mRNA-protein interactions between iron-responsive elements (IREs) and iron regulatory protein (IRP). In iron-depleted cells, IRP binds to single IREs located in the 5' untranslated regions of ferritin and eALAS mRNAs and represses translation initiation. The molecular mechanism underlying this translational repression was investigated using reconstituted, IRE-IRP-regulated, cell-free translation systems. The IRE-IRP interaction is shown to prevent the association of the 43S translation pre-initiation complex (including the small ribosomal subunit) with the mRNA. Studies with the spliceosomal protein U1A and mRNAs which harbour specific binding sites for this protein in place of an IRE furthermore reveal that the 5' termini of mRNAs are generally sensitive to repressor protein-mediated inhibition of 43S pre-initiation complex binding.

引用

页码：3882 / 3891

页数：10

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