APPLICATION OF BIOTIN-STREPTAVIDIN TECHNOLOGY IN DEVELOPING A XANTHINE BIOSENSOR BASED ON A SELF-ASSEMBLED PHOSPHOLIPID MEMBRANE

被引：43

作者：

REHAK, M

SNEJDARKOVA, M

OTTO, M

机构：

[1] Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences

来源：

BIOSENSORS & BIOELECTRONICS | 1994年 / 9卷 / 4-5期

关键词：

AMPEROMETRIC BIOSENSOR; BILAYER LIPID MEMBRANE; XANTHINE OXIDASE;

D O I：

10.1016/0956-5663(94)80033-2

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We have designed an amperometric xanthine minibiosensor based on a supported biotinylated phospholipid membrane. To this membrane streptavidin-modified xanthine oxidase was coupled. The fabricated biosensor corresponds in shape and size to a needle of 0.3 mm in diameter. The assay is based on the electrochemical detection of enzymatically generated hydrogen peroxide. The response to xanthine was linear up to 1 mmol.l-1 with a detection limit of 0.02 mmol.-1. The response time was less than one minute and the biosensor was stable for at least five days.

引用

页码：337 / 341

页数：5

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