ENZYME-BASED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY STATIONARY PHASES AS METABOLIC REACTORS - IMMOBILIZATION OF NON-SOLUBILIZED RAT-LIVER MICROSOMES ON AN IMMOBILIZED ARTIFICIAL MEMBRANE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY SUPPORT

被引：16

作者：

ALEBICKOLBAH, T ^{[1
]}

WAINER, IW ^{[1
]}

机构：

[1] MCGILL UNIV,MONTREAL GEN HOSP,DEPT ONCOL,1650 CEDAR AVE,ROOM B7113,MONTREAL H3G 1A4,QUEBEC,CANADA

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 646卷 / 02期

关键词：

D O I：

10.1016/0021-9673(93)83341-O

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Non-solubilized rat liver microsomes have been non-covalently immobilized on an immobilized artificial membrane (IAM) HPLC stationary phase. The microsomes were immobilized on the IAM interphase of both loose IAM support and in a 1 cm x 3 mm I.D. HPLC column filled with IAM stationary phase. The activity of the immobilized microsomes was assessed by following the formation of 7-hydroxycoumarin (7-OHC) from the O-deethylation of 7-ethoxycoumarin. The 7-OHC was quantified by either HPLC analysis of an aliquot from an incubation mixture containing the loose microsome-IAM support or directly using the chromatogram from the microsome-IAM HPLC column. Both forms of the IAM-immobilized microsomes were active. The result of this study indicate that the IAM stationary phase can be used to produce immobilized microsomal reactors which can be used in HPLC systems for direct on-line determination of biosynthetic and metabolic processes.

引用

页码：289 / 295

页数：7

共 11 条

[1] ENZYME-BASED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY SUPPORTS AS PROBES OF ENZYME-ACTIVITY AND INHIBITION - THE IMMOBILIZATION OF TRYPSIN AND ALPHA-CHYMOTRYPSIN ON AN IMMOBILIZED ARTIFICIAL MEMBRANE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY SUPPORT [J].