MICROTUBULES IN THE FISSION YEAST SCHIZOSACCHAROMYCES-POMBE CONTAIN ONLY THE TYROSINATED FORM OF ALPHA-TUBULIN

被引:27
作者
ALFA, CE [1 ]
HYAMS, JS [1 ]
机构
[1] UNIV LONDON UNIV COLL, DEPT BIOL, DARWIN BLDG, GOWER ST, LONDON WC1E 6BT, ENGLAND
来源
CELL MOTILITY AND THE CYTOSKELETON | 1991年 / 18卷 / 02期
关键词
TUBULIN; DETYROSINATION; CDC MUTANTS; D2O;
D O I
10.1002/cm.970180203
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The state of tubulin tyrosination in the fission yeast Schizosaccharomyces pombe was investigated using a combination of indirect immunofluorescence microscopy and Western blotting. Antibodies specific for the tyrosinated form of alpha-tubulin stained all microtubule arrays in wild type cells and recognized the two alpha-tubulin polypeptides in Western blots of cell extracts enriched for tubulin by DEAE-Sephadex chromatography. Antisera that specifically recognised the detyrosinated, glu, form, on the other hand, gave consistently negative results, both in cells undergoing rapid exponential growth and in those allowed to accumulate in stationary phase. Neither the "ageing" of microtubules, by arresting cells at different points (late G1 or G2/M) in the cell division cycle, nor stabilising them, using D2O, lead to any detectable tubulin detyrosination. These results suggest that S. pombe lacks the carboxypeptidase that carries out the tubulin detyrosination reaction. This is the first report of an organism that possesses the correct C-terminal alpha-tubulin sequence yet fails to carry out this post-translational modification. The implication of this novel finding for the biological role of these events is discussed.
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页码:86 / 93
页数:8
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