STEREOSPECIFICITY OF HYDROGEN TRANSFER IN THE SACCHAROPINE DEHYDROGENASE REACTION

被引：13

作者：

FUJIOKA, M

TAKATA, Y

机构：

[1] Department of Biochemistry, Toyama Medical and Pharmaceutical University School of Medicine, Toyama, 930-01

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 570卷 / 01期

关键词：

Hydrogen transfer; Sacchararopine dehydrogenase; Stereospecificity;

D O I：

10.1016/0005-2744(79)90215-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The stereospecificity of hydrogen transfer in the synthesis of saccharopine from α-ketoglutarate and l-lysine catalyzed by saccharopine dehydrogenase (N5-(1,3-dicarboxypropyl)-L-lysine: NAD oxidoreductase (L-lysine-forming) EC 1.5.1.7) was examined by using [4A-3H]- and [4B-3H] NADH. The enzyme showed the A-stereospecificity. The NMR analysis of the saccharopine prepared with [4A-2H]NADH revealed that the label was incorporated into the C-2 of the glutaryl moiety. © 1979.

引用

页码：210 / 212

页数：3

共 12 条

[1] STEREOCHEMISTRY OF HYDROGEN TRANSFER TO NAD CATALYZED BY (S)ALANINE DEHYDROGENASE FROM BACILLUS-SUBTILIS [J].