RHEOLOGICAL CHARACTERISTICS OF CELL-SUSPENSION AND CELL-CULTURE OF PERILLA-FRUTESCENS

被引：27

作者：

ZHONG, JJ ^{[1
]}

SEKI, T ^{[1
]}

KINOSHITA, SI ^{[1
]}

YOSHIDA, T ^{[1
]}

机构：

[1] OSAKA UNIV, FAC ENGN, INT CTR COOPERAT RES BIOTECHNOL, 2-1 YAMADA OKA, SUITA, OSAKA 565, JAPAN

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1992年 / 40卷 / 10期

关键词：

RHEOLOGY; PERILLA-FRUTESCENS; PLANT CELL CULTURE; CELL SUSPENSION; BINGHAM FLUID; MORPHOLOGY;

D O I：

10.1002/bit.260401015

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Physical properties such as viscosity, fluid dynamic behavior of cell suspension, and size distribution of cell aggregates of a plant, Perilla frutescens, cultured in a liquid medium were studied. As a result of investigations using cells harvested after 12 days of cultivation in a flask, it was found that the apparent viscosity of the cell suspension did not change with any variation of cell concentration below 5 g dry cell/L but markedly increased when the cell concentration increased over 12.8 g dry cell/L. The cell suspension exhibited the characteristics of a Bingham plastic fluid with a small yield stress. The size of cell aggregates in the range 74 to 500 mum did not influence the rheological characteristics of the cell suspension. The rheological characteristics of cultivation mixtures of P. frutescens cultivated in a flask and in a bioreactor were also investigated. The results showed that the flow characteristics of the cell culture could be described by a Bingham plastic model. At the later stage of cultivation, the apparent viscosity increased steadily, even though the biomass concentration (by dry weight) decreased, due to the increase of individual cell size.

引用

页码：1256 / 1262

页数：7

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