ISOLATION AND CHARACTERIZATION OF ISOMALTULOSE-PRODUCING AND TREHALULOSE-PRODUCING BACTERIA FROM THAILAND SOIL

被引:32
作者
TSUYUKI, K
SUGITANI, T
MIYATA, Y
EBASHI, T
NAKAJIMA, Y
机构
[1] Research and Development Department, Mitsui Sugar Co., Ltd., Kawasaki-ku, Kawasaki 210
关键词
D O I
10.2323/jgam.38.483
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The bacterial screening to get the strains that produce isomaltulose (palatinose, 6-O-alpha-D-glucopyranosyl-D-fructose) and trehalulose (1-O-alpha-D-glucopyranosyl-D-fructose) from sucrose, were carried out in Thailand. Many samples such as soil, fruits, etc., were obtained mainly from the northeastern area of Thailand. And several strains named MX-2, -4, -10, -11, and -13 were isolated from these samples. These strains, which were facultative anaerobic, gram-negative and rod-shaped bacteria, belonged to the genus Klebsiella: K. planticola (MX-2, -4, -10, and -11), and K. terrigena (MX-13). The strain MX-10 produced isomaltulose and trehalulose from sucrose, and the yields of isomaltulose and trehalulose were 65 and 30% as against sucrose, respectively, when 98% of sucrose was transformed. It is interesting that the trehalulose yields were higher in the reactions by the strain MX-10 than those by Protaminobacter rubrum (9% of trehalulose and 86% of isomaltulose). The results obtained by using immobilized cells of MX-10 were described.
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页码:483 / 490
页数:8
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