QUANTITATIVE HIGH-PRESSURE LIQUID-CHROMATOGRAPHY OF 6-THIOGUANINE IN BIOLOGICAL-FLUIDS

被引：7

作者：

BREITHAUPT, H

GOEBEL, G

机构：

[1] Med. Klin., Justus Liebig-Univ., D-6300 Giessen, Germany

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 1981年 / 19卷 / 10期

关键词：

Biomedical engineering;

D O I：

10.1093/chromsci/19.10.496

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The determination of 6-thioguanine in human plasma and urine is described. By using isocratic reversed-phase high pressure liquid chromatography (HPLC) the purine analogues, 6-thioguanine, guanine, 8-azaguanine, allopurinol, oxipurinol, and uric acid, are completely separated within 8 min. The sensitivity of the assay for 6-thioguanine is 0. 2 mu g/ml (1. 2 multiplied by 10** minus **6 M) with a precision of 3. 7%. The applicability for clinical monitoring in a patient's plasma and urine is demonstrated.

引用

页码：496 / 499

页数：4

共 16 条

[1] QUANTITATIVE-DETERMINATION OF METABOLITES OF 6-MERCAPTOPURINE IN BIOLOGICAL-MATERIALS .1. SEPARATION METHOD FOR PURINE AND 6-THIOPURINE BASES AND NUCLEOSIDES USING HIGH-PRESSURE LIQUID CATION-EXCHANGE CHROMATOGRAPHY [J].