DETERMINATION OF IVERMECTIN RESIDUES IN THE TISSUES OF ATLANTIC SALMON (SALMO-SALAR) USING HPLC WITH FLUORESCENCE DETECTION

被引：25

作者：

KENNEDY, DG

CANNAVAN, A

HEWITT, SA

RICE, DA

BLANCHFLOWER, WJ

机构：

[1] Department of Agriculture for Northern Ireland, Veterinary Sciences Division, Belfast, BT4 3SD, Stoney Road, Stormont

[2] Nutrition Services International, Randalstown, BT41 2EB

来源：

FOOD ADDITIVES AND CONTAMINANTS | 1993年 / 10卷 / 05期

关键词：

IVERMECTIN; RESIDUES; SALMON;

D O I：

10.1080/02652039309374181

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A sensitive, accurate and reproducible assay for the determination of residual concentrations of ivermectin in the tissues of farmed Atlantic salmon is described. The assay employs the clean-up of an acetonitrile extract of salmon tissue on a Bond-Elut Cg column, followed by derivatization with trifluoroacetic acid anhydride. Following separation using HPLC, 22,23-dihydroavermectin B1a is determined using fluorescence detection. The limit of detection of the assay is 1 ng per g tissue. Liver, taken from fish treated experimentally with ivermectin, was found to contain higher concentrations of 22,23-dihydroavermectin B1a than any other tissue examined. The half-life of 22,23-dihydroavermectin B1a was similar in all tissues studied, ranging from 89 to 98 degree days.

引用

页码：579 / 584

页数：6

共 13 条

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