EFFECT OF ACUTE BILE-ACID POOL DEPLETION ON TOTAL AND IONIZED CALCIUM CONCENTRATIONS IN HUMAN BILE

Although calcium salts are important components of gallstones, there are few data on the total and ionized calcium content of human bile. Therefore, in 14 fasting T-tube patients studied 7-11 days after cholecystectomy, we measured bile flow, bile acid [BA], total [Ca-TOT] and free ionized [Ca++] calcium concentrations, in 20-30 min bile collections during acute BA pool depletion induced by 6-8 h of continuous bile drainage. During washout of the BA pool there were parallel falls in bile flow, BA output and total calcium output (correlation coefficients ranging from 0.59 to 0.99; P < 0.02-0.001). In 12 of the 14 patients, [Ca-TOT] also fell (from 1.84 +/- 0.29 to 1.32 +/- 0.34 mmol L(-1)) in parallel with [BA] (from 34.0 +/- 14.0 to 8.2 +/- 8.0 mmol L(-1) r=0.75-0.98; P<0.005).In contrast, biliary [Ca++] remained virtually unchanged. These data suggest that the BAs are linked to the bound, rather than to the free, ionized, fraction of biliary calcium, which is consistent with in vivo calcium binding by BAs. A model is proposed in which BA-induced biliary calcium secretion results from (i) bile acid-induced water flow via solvent drag; and (ii) calcium binding in the bile canaliculus by bile acids, which induces paracellular diffusion of Ca++, thereby maintaining [Ca++] independent of [BA].