THE CHEMISTRY OF ENZYME AND PROTEIN IMMOBILIZATION WITH GLUTARALDEHYDE

Immobilization of proteins to solid matrices has been performed for the last thirty years and has provided numerous examples of successful preparations with use in enzyme reactors, sensor preparation and immunodiagnostics. Among the arsenal of coupling reagents and procedures, glutaraldehyde plays a critical role due to its reliability and ease of use. It displays a complex chemistry that is transparent to most practitioners of immobilization. In this article we detail the structure and reactivity of glutaraldehyde protein immobilization.