ISOLATION OF HUMAN LEUKOCYTE GRANULES USING COLLOIDAL SILICA-POLYSACCHARIDE DENSITY GRADIENTS

被引：39

作者：

OLSSON, I

机构：

[1] Department of Physiological Chemistry, Research Laboratory of the Hospital, University of Lund, Lund

来源：

EXPERIMENTAL CELL RESEARCH | 1969年 / 54卷 / 03期

关键词：

D O I：

10.1016/0014-4827(69)90210-9

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The cytoplasmic granules from leukocytes have been centrifuged in colloidal silica at physiological pH and osmolality. The granules were obtained in the density range from 1.24-1.28 g/ml and contained all acid phosphatase, β-glucuronidase and β-galactosidase activity. Electron microscopical studies on the washed fractions revealed intact granules contaminated with some silica. Alkaline phosphatase containing organelles did not show a reproduceable pattern but separated from those containing acid hydrolases. © 1969.

引用

页码：325 / &

共 19 条

[1] TISSUE FRACTIONATION STUDIES .18. RESOLUTION OF MITOCHONDRIAL FRACTIONS FROM RAT LIVER INTO 3 DISTINCT POPULATIONS OF CYTOPLASMIC PARTICLES BY MEANS OF DENSITY EQUILIBRATION IN VARIOUS GRADIENTS [J].