CHARACTERIZATION OF A 5-METHYLTRYPTOPHAN RESISTANT STRAIN OF CATHARANTHUS-ROSEUS CULTURED-CELLS

被引：30

作者：

SCOTT, AI

MIZUKAMI, H

LEE, SL

机构：

[1] Department of Chemistry, Texas A and M University, College Station

来源：

PHYTOCHEMISTRY | 1979年 / 18卷 / 05期

基金：

美国国家卫生研究院;

关键词：

ajmalicine; Apocynaceae; Catharanthus roseus; indole alkaloids; selection; suspension cultures; tryptophan biosynthesis;

D O I：

10.1016/0031-9422(79)80017-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Strains of Catharanthus roseus suspension cells resistant to growth inhibition by various tryptophan analogs were selected. Tryptophan synthetase and anthranilate synthetase from the resistant cells differed from the normal cell enzymes by being more resistant to feedback inhibition by tryptophan. Though these altered enzymes allowed the free tryptophan level of the resistant cells to be 3-40 times higher than that of normal cells, the accumulation of tryptamine or ajmalicine could not be detected in the resistant cells. © 1979.

引用

页码：795 / 798

页数：4

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