ANTIBODIES DIRECTED AGAINST ANTIGENS ON THE ENDOTHELIUM OF PERITUBULAR CAPILLARIES IN PATIENTS WITH REJECTING RENAL-ALLOGRAFTS

被引:107
作者
PAUL, LC
VANES, LA
VANROOD, JJ
VANLEEUWEN, A
DELARIVIERE, GB
DEGRAEFF, J
机构
[1] STATE UNIV LEIDEN HOSP, DEPT IMMUNOHAEMATOL, LEIDEN, NETHERLANDS
[2] STATE UNIV LEIDEN HOSP, DEPT PATHOL, LEIDEN, NETHERLANDS
关键词
D O I
10.1097/00007890-197903000-00007
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
This study was undertaken to examine the humoral immune response against endothelial antigens of the donor kidney in human renal allograft recipients. Sera from 61 transplant recipients who received 62 grafts were studied for the presence of circulating endothelial antibodies (CEAb) using an indirect immunofluorescence technique with a pretransplant biopsy of the graft as a substrate. IgG antibodies directed against the endothelium of peritubular capillaries were found in the sera of 6 of the 10 patients with graft rejection within 7 weeks after transplantation, whereas these antibodies were not found in the absence of rejection (P < 0.001). Immunofluorescence studies of post-transplant biopsies showed IgG along the endothelium of peritubular capillaries only in the grafts of patients with CEAb. Eluates from these grafts contained IgG antibodies that bound to the endothelium of the donor as shown by the indirect immunofluorescence téchnique. Absorption of endothelial antibody (EAb)-positive sera with human platelets or Wistar strain rat erythrocytes showed that the EAb were not directed against serologically defined HLA antigens or against heterophile antigens on rat erythrocytes. We conclude from this study that the presence of antibodies directed against endothelial antigens is associated with poor graft prognosis and that these antibodies may be responsible for the rejection process. © 1979 by The Williams & Wilkins Co.
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页码:175 / 179
页数:5
相关论文
共 27 条
  • [1] ABBAS AK, 1974, AM J PATHOL, V75, P271
  • [2] ABBAS AK, 1974, AM J PATHOL, V75, P255
  • [3] CROSS-LINKING OF PROTEINS WITH GLUTARALDEHYDE AND ITS USE FOR PREPARATION OF IMMUNOADSORBENTS
    AVRAMEAS, S
    TERNYNCK, T
    [J]. IMMUNOCHEMISTRY, 1969, 6 (01): : 53 - +
  • [4] HUMAN RENAL ALLOGRAFTS - ROLE OF VASCULAR INJURY IN EARLY GRAFT FAILURE
    BUSCH, GJ
    REYNOLDS, ES
    GALVANEK, EG
    BRAUN, WE
    DAMMIN, GJ
    [J]. MEDICINE, 1971, 50 (01) : 29 - +
  • [5] CERILLI J, 1977, SURGERY, V81, P132
  • [6] DEPOSITION OF IMMUNOREACTANTS IN PRESERVED HUMAN KIDNEYS
    GILBOA, N
    DURANTE, D
    YOKOTA, K
    WEIL, R
    MCINTOSH, RM
    [J]. TRANSPLANTATION, 1977, 24 (04) : 304 - 307
  • [7] HUMAN MIXED LYMPHOCYTE-ENDOTHELIUM CULTURE INTERACTION
    HIRSCHBERG, H
    EVENSEN, SA
    HENRIKSEN, T
    THORSBY, E
    [J]. TRANSPLANTATION, 1975, 19 (06) : 495 - 504
  • [8] HOLBOROW EJ, 1967, HDB EXPT IMMUNOLOGY, P571
  • [9] IMMUNOLOGICAL STUDIES ON ELUATES FROM HUMAN KIDNEY GRAFTS
    JEANNET, M
    LAMBERT, PH
    [J]. CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY, 1975, 4 (04): : 478 - 488
  • [10] ROLE OF ANTI-GLOMERULAR BASEMENT MEMBRANE ANTIBODY IN PATHOGENESIS OF HUMAN GLOMERULONEPHRITIS
    LERNER, RA
    GLASSOCK, RJ
    DIXON, FJ
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1967, 126 (06) : 989 - &