3-AZIDO-L-TYROSINE AS A PHOTOINHIBITOR OF TUBULIN - TYROSINE LIGASE ROLE OF THIOL-GROUPS

被引：9

作者：

COUDIJZER, K ^{[1
]}

JONIAU, M ^{[1
]}

机构：

[1] KATHOLIEKE UNIV LEUVEN,INTERDISCIPLINARY RES CTR,CAMPUS KORTRIJK,B-8500 KORTRIJK,BELGIUM

来源：

FEBS LETTERS | 1990年 / 268卷 / 01期

关键词：

3-Azido-L-tyrosine; Photoinaetivation; Posttranslational modification; Thiol group; Tubulin; Tubulin:tyrosine ligase;

D O I：

10.1016/0014-5793(90)80981-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

We have synthesized the photoactivatable probes 3-azido-L-tyrosine andp-azido-L-phenylalanine and studied their capacity to inhibit the incorporation of[3H]tyrosine into tubulin catalyzed by tubulin:tyrosine ligase. Without illumination, only 3-azido-L-tyrosine reversibly inhibits the enzyme. Upon illumination, both reagents irreversibly photoinactivate the enzyme in a similar way. The ligase can be protected against photoinactivation by reversibly blocking essential thiol groups with pCMB during illumination. © 1990.

引用

页码：95 / 98

页数：4

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