3-DIMENSIONAL READOUT OF FLASH X-RAY IMAGES OF LIVING SPERM IN WATER BY ATOMIC-FORCE MICROSCOPY

被引：80

作者：

TOMIE, T ^{[1
]}

SHIMIZU, H ^{[1
]}

MAJIMA, T ^{[1
]}

YAMADA, M ^{[1
]}

KANAYAMA, T ^{[1
]}

KONDO, H ^{[1
]}

YANO, M ^{[1
]}

ONO, M ^{[1
]}

机构：

[1] NIKON INC,TSUKUBA LAB,TSUKUBA,IBARAKI 30026,JAPAN

来源：

SCIENCE | 1991年 / 252卷 / 5006期

关键词：

D O I：

10.1126/science.2024121

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The imaging of living specimens in water by x-ray microscopy can be greatly enhanced with the use of an intense flash x-ray source and sophisticated technologies for reading x-ray images. A subpicosecond x-ray pulse from a laser-produced plasma was used to record the x-ray image of living sea urchin sperm in an x-ray resist. The resist relief was visualized at high resolution by atomic-force microscopy. Internal structure of the sperm head was evident, and the carbon density in a flagellum was estimated from the relief height.

引用

页码：691 / 693

页数：3

共 14 条

[1] A REINVESTIGATION OF CROSS-SECTIONS OF CILIA
ALLEN, RD
[J]. JOURNAL OF CELL BIOLOGY, 1968, 37 (03) : 825 - +
[2] ATOMIC FORCE MICROSCOPE
BINNIG, G
QUATE, CF
GERBER, C
[J]. PHYSICAL REVIEW LETTERS, 1986, 56 (09) : 930 - 933
[3] Cheng P.C., 1987, XRAY MICROSCOPY INST, P65
[4] EASON RW, 1985, ANN REPORT
[5] DIRECT IMAGING OF LIVE HUMAN-PLATELETS BY FLASH X-RAY MICROSCOPY
FEDER, R
BANTON, V
SAYRE, D
COSTA, J
BALDINI, M
KIM, B
[J]. SCIENCE, 1985, 227 (4682) : 63 - 64
[6] HIGH-RESOLUTION SOFT-X-RAY MICROSCOPY
FEDER, R
SPILLER, E
TOPALIAN, J
BROERS, AN
GUDAT, W
PANESSA, BJ
ZADUNAISKY, ZA
SEDAT, J
[J]. SCIENCE, 1977, 197 (4300) : 259 - 260
[7] GIUDICE G, 1987, SEA URCHIN EMBRYO, P23
[8] JACOBSEN C, 1988, XRAY MICROSCOPY, V2, P253
[9] KIRZ J, 1984, XRAY MICROSCOPY, P262
[10] WAVELENGTH CHOICE FOR SOFT-X-RAY LASER HOLOGRAPHY OF BIOLOGICAL SAMPLES
LONDON, RA
ROSEN, MD
TREBES, JE
[J]. APPLIED OPTICS, 1989, 28 (16): : 3397 - 3404

← 1 2 →