NEURONS IN THE RAT MEDULLA-OBLONGATA CONTAINING NEUROPEPTIDE-Y-LIKE, ANGIOTENSIN-II-LIKE, OR GALANIN-LIKE IMMUNOREACTIVITY PROJECT TO THE PARABRACHIAL NUCLEUS

被引:50
作者
KRUKOFF, TL [1 ]
VU, T [1 ]
HARRIS, KH [1 ]
AIPPERSBACH, S [1 ]
JHAMANDAS, JH [1 ]
机构
[1] UNIV ALBERTA,FAC MED,DEPT MED NEUROL,EDMONTON T6G 2H7,ALBERTA,CANADA
关键词
D O I
10.1016/0306-4522(92)90130-T
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Projections from the medulla to the parabrachial complex of the rat were examined for their content of neuropeptide Y-, angiotensin II- or galanin-like immunoreactivity using combined retrograde tracing and immunohistochemical techniques. Rhodamine-labelled latex microspheres were stereotaxically injected into discrete nuclei of the parabrachial complex. After survival of two to five days, colchicine (100-mu-g in 10-mu-l saline) was injected into the cisterna magna. One day later, rats were perfused and the brainstems were prepared for visualization of the retrograde tracer and immunoreactivity of one of the three peptides. Retrograde labelling verified that the area postrema, nucleus of the tractus solitarius, caudal spinal nucleus of the trigeminal nerve, parvocellular reticular nucleus, and ventrolateral medulla including the rostral ventrolateral medulla and nucleus paragigantocellularis project to the lateral parabrachial and Kolliker-Fuse nuclei. While most projections were primarily ipsilateral, a small proportion of the projections from the ventrolateral medulla was bilateral. Neurons containing neuropeptide Y-like immunoreactivity were found in the caudal and intermediate nucleus of the tractus solitarius, dorsal to the lateral reticular nucleus and in the nucleus paragigantocellularis. After bilateral microsphere injections into the lateral parabrachial and Kolliker-Fuse nuclei, double-labelled neurons were found dorsal to the lateral reticular nucleus of caudal and intermediate medullary levels, at the ventral surface of the medulla at intermediate levels and in the nucleus paragigantocellularis at rostral levels. Neurons with angiotensin II-like immunoreactivity were observed at the dorsomedial border of the caudal and intermediate nucleus of the tractus solitarius, in the area postrema and in the lateral reticular nucleus and nucleus paragigantocellularis. Of these neurons, small numbers in the nucleus of the tractus solitarius and ventrolateral medulla also projected to the lateral parabrachial and Kolliker-Fuse nuclei. Neurons containing galanin-like immunoreactivity were found in the caudal nucleus of the tractus solitarius, the area postrema, the spinal trigeminal nucleus, the raphe nuclei (pallidus and obscurus), the nucleus paragigantocellularis and dorsal to the lateral reticular nucleus. Of these cells, double-labelled neurons were found in the commissural and medial subdivisions of the caudal nucleus of the tractus solitarius and in the rostral ventrolateral medulla including the ventral surface and the nucleus paragigantocellularis. The results suggest that neuropeptide Y, angiotensin II and galanin may serve as neurochemical messengers in pathways from the medulla to the parabrachial complex. The location of double-labelled neurons suggests that the information relayed by these neurons is related to autonomic activity.
引用
收藏
页码:175 / 184
页数:10
相关论文
共 55 条
  • [1] NEUROPEPTIDE Y-LIKE IMMUNOREACTIVITY IN THE BRAIN-STEM RESPIRATORY NUCLEI OF THE CAT
    AGUIRRE, JA
    COVENAS, R
    DAWIDMILNER, MS
    ALONSO, JR
    GARCIAHERDUGO, G
    GONZALEZBARON, S
    [J]. BRAIN RESEARCH BULLETIN, 1989, 23 (03) : 201 - 207
  • [2] IMMUNOCYTOCHEMICAL STUDY OF ANGIOTENSIN-II FIBERS AND CELL-BODIES IN THE BRAIN-STEM RESPIRATORY AREAS OF THE CAT
    AGUIRRE, JA
    COVENAS, R
    CROIX, D
    ALONSO, JR
    NARVAEZ, JA
    TRAMU, G
    GONZALEZBARON, S
    [J]. BRAIN RESEARCH, 1989, 489 (02) : 311 - 317
  • [3] NEURONS WITHIN CHEMOSENSITIVE AREA ON VENTRAL SURFACE OF BRAIN-STEM WHICH PROJECT TO INTERMEDIOLATERAL COLUMN
    AMENDT, K
    CZACHURSKI, J
    DEMBOWSKY, K
    SELLER, H
    [J]. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1978, 375 (03): : 289 - 292
  • [4] NEURONS OF C1 AREA MEDIATE CARDIOVASCULAR-RESPONSES INITIATED FROM VENTRAL MEDULLARY SURFACE
    BENARROCH, EE
    GRANATA, AR
    RUGGIERO, DA
    PARK, DH
    REIS, DJ
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1986, 250 (05): : R932 - R945
  • [5] BETRAND F, 1971, J ENRUOPHYSIOL, V84, P189
  • [6] SPINAL INPUT TO THE PARABRACHIAL NUCLEUS IN THE CAT
    BLOMQVIST, A
    MA, W
    BERKLEY, KJ
    [J]. BRAIN RESEARCH, 1989, 480 (1-2) : 29 - 36
  • [7] CARDIOVASCULAR EFFECTS OF ANGIOTENSIN-(1-7) INJECTED INTO THE DORSAL MEDULLA OF RATS
    CAMPAGNOLESANTOS, MJ
    DIZ, DI
    SANTOS, RAS
    KHOSLA, MC
    BROSNIHAN, KB
    FERRARIO, CM
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1989, 257 (01): : H324 - H329
  • [8] SPINAL AND TRIGEMINAL DORSAL HORN PROJECTIONS TO THE PARABRACHIAL NUCLEUS IN THE RAT
    CECHETTO, DF
    STANDAERT, DG
    SAPER, CB
    [J]. JOURNAL OF COMPARATIVE NEUROLOGY, 1985, 240 (02) : 153 - 160
  • [9] PARABRACHIAL UNITS RESPONDING TO STIMULATION OF BUFFER NERVES AND FOREBRAIN IN THE CAT
    CECHETTO, DF
    CALARESU, FR
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1983, 245 (06): : R811 - R819
  • [10] FUNCTION OF THE VENTROLATERAL MEDULLA IN THE CONTROL OF THE CIRCULATION
    CIRIELLO, J
    CAVERSON, MM
    POLOSA, C
    [J]. BRAIN RESEARCH REVIEWS, 1986, 11 (04) : 359 - 391