SENSITIVE DETERMINATION OF SALMON-CALCITONIN, BY MEANS OF PRECOLUMN DERIVATIZATION, HPLC AND FLUOROMETRIC-DETERMINATION

被引：7

作者：

FUKUDA, T ^{[1
]}

ISHIKAWA, K ^{[1
]}

IMAI, K ^{[1
]}

机构：

[1] UNIV TOKYO,FAC PHARMACEUT SCI,DEPT ANALYT CHEM,BUNKYO KU,TOKYO 113,JAPAN

来源：

BIOMEDICAL CHROMATOGRAPHY | 1995年 / 9卷 / 01期

关键词：

D O I：

10.1002/bmc.1130090111

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high sensitive analytical method for salmon calcitonin (sCT) was established by means of derivatization with a fluorescence labelling agent, DBD-F, followed by HPLC separation and fluorescence detection at 558 nm with excitation at 430 nm. Under optimized conditions (DBD-F, 0.1 M berate buffer (pH 8.5):acetonitrile (70:30, v/v)), three molecules of DBD-F reacted with one sCT molecule in the presence of 1 mM sodium dodccyl sulphate (SDS) at 50 degrees C for 3 h. The detection limit for the sCT derivative was 20 fmol. A linear relationship was obtained between the amount of sCT on column and the peak heights in the range of 35-8000 fmol.

引用

页码：52 / 55

页数：4

共 9 条

[1] A VALIDATED HPLC ASSAY FOR SALMON-CALCITONIN ANALYSIS - COMPARISON OF HPLC AND BIOLOGICAL ASSAY [J].

BUCK, RH ;

MAXL, F .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1990, 8 (8-12) :761-769

[2] A RAPID AND SENSITIVE DETERMINATION OF SALMON-CALCITONIN IN SOLUTIONS CONTAINING BOVINE SERUM-ALBUMIN OR GELATIN [J].

FUKUDA, T ;

YUN, A ;

IMAI, K .

BIOMEDICAL CHROMATOGRAPHY, 1993, 7 (04) :229-230

[3] INFLUENCE OF THE MOBILE PHASE ON SALMON-CALCITONIN ANALYSIS BY REVERSED-PHASE LIQUID-CHROMATOGRAPHY [J].

LEE, IH ;

POLLACK, S ;

HSU, SH ;

MIKSIC, JR .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1991, 29 (04) :136-140

[4] BIOANALYSIS OF THE NEUROPEPTIDE DES-ENKEPHALIN-GAMMA-ENDORPHIN BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ONLINE SAMPLE PRETREATMENT USING GEL-PERMEATION AND SOLID-PHASE ISOLATION [J].

STEGEHUIS, DS ;

TJADEN, UR ;

VANDENBELD, CMB ;

VANDERGREEF, J .

JOURNAL OF CHROMATOGRAPHY, 1991, 549 (1-2) :185-193

[5] SENSITIVE DETERMINATION OF N-TERMINAL PROLYL PEPTIDES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH LASER-INDUCED FLUORESCENCE DETECTION [J].

TOYOOKA, T ;

ISHIBASHI, M ;

TERAO, T .

JOURNAL OF CHROMATOGRAPHY A, 1994, 661 (1-2) :105-112

[6] EVALUATION OF BENZOFURAZAN DERIVATIVES AS FLUOROGENIC REAGENTS FOR THIOLS AND AMINES USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

TOYOOKA, T ;

SUZUKI, T ;

SAITO, Y ;

UZU, S ;

IMAI, K .

ANALYST, 1989, 114 (10) :1233-1240

[7] ISOLATION AND CHARACTERIZATION OF CYSTEINE-CONTAINING REGIONS OF PROTEINS USING 4-(AMINOSULFONYL)-7-FLUORO-2,1,3-BENZOXADIAZOLE AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

TOYOOKA, T ;

IMAI, K .

ANALYTICAL CHEMISTRY, 1985, 57 (09) :1931-1937

[8] FLUOROGENIC REAGENT FOR THIOLS - 4-(N,N-DIMETHYLAMINOSULPHONYL)-7-FLUORO-2,1,3-BENZOXADIAZOLE [J].

TOYOOKA, T ;

SUZUKI, T ;

SAITO, Y ;

UZU, S ;

IMAI, K .

ANALYST, 1989, 114 (04) :413-419

[9]

UZU S, 1991, BIOMED CHROMATOGR, V3, P184

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