SIMPLIFIED METHOD FOR THE PREPARATION OF PURE UDP-GLUCOSE-C-14

被引:46
作者
TAN, AWH [1 ]
机构
[1] UNIV MINNESOTA,DEPT BIOCHEM,MINNEAPOLIS,MN 55417
关键词
UDP[[!sup]14[!/sup]C]glucose preparation;
D O I
10.1016/0304-4165(79)90146-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A two-step enzymatic synthesis of UDP[14C]glucose was described which resulted in high yield. Separation of product from labelled intermediates and other contaminants was achieved by a simple ion exchange chromatography method. © 1979.
引用
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页码:543 / 547
页数:5
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