DISTRIBUTION OF MSDNAS AMONG SEROTYPES OF ENTEROPATHOGENIC ESCHERICHIA-COLI STRAINS

被引：20

作者：

LIM, D

GOMES, TAT

MAAS, WK

机构：

[1] NYU MED CTR,DEPT MICROBIOL,NEW YORK,NY 10016

[2] ESCOLA PAULISTA MED SCH,DEPT MICROBIOL IMMUNOL & PARASITOL,BR-04023 SAO PAULO,BRAZIL

来源：

MOLECULAR MICROBIOLOGY | 1990年 / 4卷 / 10期

关键词：

D O I：

10.1111/j.1365-2958.1990.tb00548.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A genetic element, called a retron, is present in certain Escherichia coli strains. It consists of genes for the production of a covalently linked DNA‐RNA compound and a reverse transcriptase. The presence of a retron can be detected by testing for a satellite DNA band by polyacrylamide gel electrophoresis. This DNA band consists of the DNA portion of the DNA–RNA compound and is called msDNA (multicopy single‐stranded DNA). In a survey of intestinal E. coli isolates we detected msDNAs in classical enteropathogenic (EPEC) strains and in strains with aggregative adherence to tissue‐culture cells (AA), but not in enteroinvasive (EIEC) and enterotoxigenic (ETEC) strains. Among 76 EPEC strains belonging to 14 different serotypes, msDNA was found to be present in 7 serotypes. In total, five different types of msDNA were found, although within each serotype, the msDNAs were the same. These results suggest that different retrons are clonally inherited. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：1711 / 1714

页数：4

共 11 条

[1]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[2] BRANCHED RNA COVALENTLY LINKED TO THE 5' END OF A SINGLE-STRANDED-DNA IN STIGMATELLA-AURANTIACA - STRUCTURE OF MSDNA [J].