ASSEMBLY OF DOUBLE-SHELLED ROTAVIRUS-LIKE PARTICLES BY SIMULTANEOUS EXPRESSION OF RECOMBINANT VP6-PROTEIN AND VP7-PROTEIN

被引：47

作者：

SABARA, M ^{[1
]}

PARKER, M ^{[1
]}

AHA, P ^{[1
]}

COSCO, C ^{[1
]}

GIBBONS, E ^{[1
]}

PARSONS, S ^{[1
]}

BABIUK, LA ^{[1
]}

机构：

[1] VET INFECT DIS ORG,SASKATOON S7N 0W0,SASKATCHEWAN,CANADA

来源：

JOURNAL OF VIROLOGY | 1991年 / 65卷 / 12期

关键词：

D O I：

10.1128/JVI.65.12.6994-6997.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Simultaneous coinfection of Spodoptera frugiperda cells with baculovirus recombinants containing the genes for VP6 and VP7 of bovine rotavirus strain C486 resulted in the production of spherical particles resembling smooth, double-shelled rotavirus. These particles were predominantly located in the cell culture supernatant instead of being cell associated. Pretreatment of infected-cell culture supernatants with CaCl2, prior to particle purification, was found to enhance the smooth appearance of the particles. The authenticity of the proteins making up the particles was demonstrated by their reactivity with antiserum specific for double-shelled C486 rotavirus.

引用

页码：6994 / 6997

页数：4

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