IMMUNOELECTRON MICROSCOPIC LOCALIZATION OF MICROSOMAL ALANINE AMINOPEPTIDASE

被引：5

作者：

KETTMANN, U

HUMBEL, B

HOLZHAUSEN, HJ

BAHN, H

AURICH, H

机构：

[1] UNIV UTRECHT, DEPT CELL BIOL, UTRECHT, NETHERLANDS

[2] MARTIN LUTHER UNIV, INST PATHOL ANAT, O-4010 HALLE, GERMANY

来源：

ACTA HISTOCHEMICA | 1992年 / 93卷 / 01期

关键词：

D O I：

10.1016/S0065-1281(11)80233-3

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The localization of microsomal alanine aminopeptidase was investigated in the rat kidney. Resin embedding failed to demonstrate the localization of the enzyme by immunogold labelling. Using a cryo-ultramicrotomy method the enzyme could be detected on the luminal side of the brush border membrane of proximal tubular cells and to a lesser degree in their mitochondria. Furthermore, vesicular structures labelled with gold were found in the cytoplasma in the apical region of these cells.

引用

页码：333 / 340

页数：8

共 19 条

[1] RESIN DEVELOPMENT FOR ELECTRON-MICROSCOPY AND AN ANALYSIS OF EMBEDDING AT LOW-TEMPERATURE [J].