ASSOCIATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ENVELOPE GLYCOPROTEIN WITH PARTICLES DEPENDS ON INTERACTIONS BETWEEN THE 3RD VARIABLE AND CONSERVED REGIONS OF GP120

被引:35
作者
WILLEY, RL
MARTIN, MA
机构
关键词
D O I
10.1128/JVI.67.6.3639-3643.1993
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Many regions within the envelope of human immunodeficiency virus type 1 (HIV-1) that affect its structure and function have been identified. We have previously reported that the interaction of the second conserved (C2) and third variable (V3) regions of gp120 influences the ability of HIV-1 to establish a productive infection in susceptible cells. To better understand the basis for this interaction, we have conducted structure-function analyses of envelope expressed from molecular proviral clones of HIV-1 containing defined mutations in C2 and V3 that individually and in combination differentially affect envelope function. The substitution of a glutamine for an asparagine residue (Q-267) at a potential asparagine-linked glycosylation site in C2, which severely impairs virus infectivity, reduces intracellular processing of gp160 into gp120, the association of gp120 with virions, and the ability of gp120 to bind to the HIV-1 cell surface receptor protein, CD4. The change of an arginine to an isoleucine codon in V3 (1-308), in the presence of the Q-267 mutation, restores virus infectivity to near wild-type levels by increasing the amount of gp120 associated with virions as compared with the Q-267 mutant but does not compensate for the Q-267-induced processing defect. The 1-308 change in the context of the wild-type HIV-1 has no affect on processing, association, or CD4 binding. These results indicate that the impaired infectivity of the Q-267 mutant virus is due to a marked reduction in the amount of virion gp120 and suggest that the interaction of C2 and V3 stabilizes the association of gp120 with gp41.
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页码:3639 / 3643
页数:5
相关论文
共 35 条
  • [1] PRODUCTION OF ACQUIRED IMMUNODEFICIENCY SYNDROME-ASSOCIATED RETROVIRUS IN HUMAN AND NONHUMAN CELLS TRANSFECTED WITH AN INFECTIOUS MOLECULAR CLONE
    ADACHI, A
    GENDELMAN, HE
    KOENIG, S
    FOLKS, T
    WILLEY, R
    RABSON, A
    MARTIN, MA
    [J]. JOURNAL OF VIROLOGY, 1986, 59 (02) : 284 - 291
  • [2] MAJOR GLYCOPROTEIN ANTIGENS THAT INDUCE ANTIBODIES IN AIDS PATIENTS ARE ENCODED BY HTLV-III
    ALLAN, JS
    COLIGAN, JE
    BARIN, F
    MCLANE, MF
    SODROSKI, JG
    ROSEN, CA
    HASELTINE, WA
    LEE, TH
    ESSEX, M
    [J]. SCIENCE, 1985, 228 (4703) : 1091 - 1094
  • [3] TARGET CELL-SPECIFIC DETERMINANTS OF MEMBRANE-FUSION WITHIN THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GP120 3RD-VARIABLE REGION AND GP41 AMINO TERMINUS
    BERGERON, L
    SULLIVAN, N
    SODROSKI, J
    [J]. JOURNAL OF VIROLOGY, 1992, 66 (04) : 2389 - 2397
  • [4] BIOLOGICAL PROPERTIES OF A CD4 IMMUNOADHESIN
    BYRN, RA
    MORDENTI, J
    LUCAS, C
    SMITH, D
    MARSTERS, SA
    JOHNSON, JS
    COSSUM, P
    CHAMOW, SM
    WURM, FM
    GREGORY, T
    GROOPMAN, JE
    CAPON, DJ
    [J]. NATURE, 1990, 344 (6267) : 667 - 670
  • [5] DESIGNING CD4 IMMUNOADHESINS FOR AIDS THERAPY
    CAPON, DJ
    CHAMOW, SM
    MORDENTI, J
    MARSTERS, SA
    GREGORY, T
    MITSUYA, H
    BYRN, RA
    LUCAS, C
    WURM, FM
    GROOPMAN, JE
    BRODER, S
    SMITH, DH
    [J]. NATURE, 1989, 337 (6207) : 525 - 531
  • [6] EFFECTS OF MUTATIONS IN HYPERCONSERVED REGIONS OF THE EXTRACELLULAR GLYCOPROTEIN OF HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 ON RECEPTOR-BINDING
    CORDONNIER, A
    RIVIERE, Y
    MONTAGNIER, L
    EMERMAN, M
    [J]. JOURNAL OF VIROLOGY, 1989, 63 (10) : 4464 - 4468
  • [7] BIOSYNTHESIS AND PROCESSING OF HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 ENVELOPE GLYCOPROTEINS - EFFECTS OF MONENSIN ON GLYCOSYLATION AND TRANSPORT
    DEWAR, RL
    VASUDEVACHARI, MB
    NATARAJAN, V
    SALZMAN, NP
    [J]. JOURNAL OF VIROLOGY, 1989, 63 (06) : 2452 - 2456
  • [8] DUNBAY JW, 1992, J VIROL, V66, P4748
  • [9] DUNBAY JW, 1992, J VIROL, V66, P6616
  • [10] OLIGOMERIC STRUCTURE OF THE HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 ENVELOPE GLYCOPROTEIN
    EARL, PL
    DOMS, RW
    MOSS, B
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (02) : 648 - 652