TRYPANOSOMA-BRUCEI MITOCHONDRIAL CR4 GENE ENCODES AN EXTENSIVELY EDITED MESSENGER-RNA WITH COMPLETELY EDITED SEQUENCE ONLY IN BLOOD-STREAM FORMS

被引：40

作者：

CORELL, RA

MYLER, P

STUART, K

机构：

[1] SEATTLE BIOMED RES INST,SEATTLE,WA 98109

[2] UNIV WASHINGTON,DEPT PATHOBIOL,SEATTLE,WA 98195

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1994年 / 64卷 / 01期

关键词：

TRYPANOSOME; RNA EDITING; CR4; MITOCHONDRION;

D O I：

10.1016/0166-6851(94)90135-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several kinetoplastid mitochondrial genes cannot be expressed without RNA editing of their transcripts to create a functional open reading frame. We have examined one such mitochondrial gene, CR4, in Trypanosoma brucei EATRO164 and find extensive editing of transcripts in both bloodstream and procyclic life cycle stages. However, a consensus edited sequence for the entire mRNA occurs only in the bloodstream stage. The unedited CR4 transcript is 283 nucleotides in length, not including the poly(A) tail. A total of 325 uridines are inserted, and 40 uridines deleted, to create the mature mRNA which encodes a very hydrophobic protein.

引用

收藏

页码：65 / 74

页数：10

相关论文

共 47 条

[1] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[2] PROSITE - A DICTIONARY OF SITES AND PATTERNS IN PROTEINS [J].

BAIROCH, A .

NUCLEIC ACIDS RESEARCH, 1991, 19 :2241-2245

[3] PCR-BASED CDNA LIBRARY CONSTRUCTION - GENERAL CDNA LIBRARIES AT THE LEVEL OF A FEW CELLS [J].

BELYAVSKY, A ;

VINOGRADOVA, T ;

RAJEWSKY, K .

NUCLEIC ACIDS RESEARCH, 1989, 17 (08) :2919-2932

[4] AN EXTENSIVELY EDITED MITOCHONDRIAL TRANSCRIPT IN KINETOPLASTIDS ENCODES A PROTEIN HOMOLOGOUS TO ATPASE SUBUNIT-6 [J].

BHAT, GJ ;

KOSLOWSKY, DJ ;

FEAGIN, JE ;

SMILEY, BL ;

STUART, K .

CELL, 1990, 61 (05) :885-894

[5] THE 2 ATPASE 6 MESSENGER-RNAS OF LEISHMANIA-TARENTOLAE DIFFER AT THEIR 3' ENDS [J].

BHAT, GJ ;

MYLER, PJ ;

STUART, K .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 48 (02) :139-149

[6]

BHAT GJ, 1992, MOL BIOCHEM PARASIT, V52, P231, DOI 10.1016/0166-6851(92)90055-O

[7] CHIMERIC GRNA MESSENGER-RNA MOLECULES WITH OLIGO(U) TAILS COVALENTLY LINKED AT SITES OF RNA EDITING SUGGEST THAT U-ADDITION OCCURS BY TRANSESTERIFICATION [J].

BLUM, B ;

STURM, NR ;

SIMPSON, AM ;

SIMPSON, L .

CELL, 1991, 65 (04) :543-550

[8] A MODEL FOR RNA EDITING IN KINETOPLASTID MITOCHONDRIA - GUIDE RNA MOLECULES TRANSCRIBED FROM MAXICIRCLE DNA PROVIDE THE EDITED INFORMATION [J].

BLUM, B ;

BAKALARA, N ;

SIMPSON, L .

CELL, 1990, 60 (02) :189-198

[9] GUIDE RNAS IN KINETOPLASTID MITOCHONDRIA HAVE A NONENCODED 3' OLIGO(U) TAIL INVOLVED IN RECOGNITION OF THE PREEDITED REGION [J].

BLUM, B ;

SIMPSON, L .

CELL, 1990, 62 (02) :391-397

[10]

CABOT EL, 1989, COMPUT APPL BIOSCI, V5, P233

← 1 2 3 4 5 →