EFFECT OF PINACIDIL ON RAT HEARTS UNDERGOING HYPOTHERMIC CARDIOPLEGIA

We studied the effect of pinacidil, a potassium-channel opener, on the hemodynamic, biochemical, and ultrastructural changes in rat hearts undergoing hypothermic cardioplegia. Fifty-four male Wistar rats weighing 250 to 300 g were used. Isolated hearts were prepared for modified Langendorff circulation in the working mode using modified Krebs-Henseleit bicarbonate solution bubbled with a 95% O-2 and 5% CO2 gas mixture. Eighty minutes of cardioplegia at 25 degrees C was followed by normothermic reperfusion for 30 minutes. Pinacidil, 5, 10, or 50 mu mol/L added to the cardioplegic solution, did not affect heart rate, but it significantly improved the recovery of aortic flow as compared with controls (88.1% +/- 4.3 [5 mu mol/L]; 83.2% +/- 8.5% [10 mu mol/L]; 90.3% +/- 5.3% [50 mu mol/L] compared with 55.6 +/- 4.3% [control]; p < 0.05). Administration of pinacidil during reperfusion did not further enhance the recovery of aortic now. The dose-response curve of aortic flow to the pinacidil concentrations was fiat from 5 to 50 mu mol/L. However, preservation of myocardial adenosine triphosphate and calcium concentrations and mitochondrial morphology suggested that the optimal concentration of pinacidil cardioplegia is 10 mu mol/L.