EFFECTS OF DNA POLYMER LENGTH ON ITS ADSORPTION TO SOILS

被引：125

作者：

OGRAM, AV ^{[1
]}

MATHOT, ML ^{[1
]}

HARSH, JB ^{[1
]}

BOYLE, J ^{[1
]}

PETTIGREW, CA ^{[1
]}

机构：

[1] PROCTER & GAMBLE CO, DEPT ENVIRONM SCI, CINCINNATI, OH 45217 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1994年 / 60卷 / 02期

关键词：

D O I：

10.1128/AEM.60.2.393-396.1994

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Three different DNA fragments ranging in size from 2.69 kbp (1.75 MDa) to 23 kbp (14.95 MDa) were used as tracers to study the adsorption of polydisperse solutions of calf thymus DNA to eight model soils. The adsorption of the three tracers to all soils was described by the Freundlich adsorption model, with adsorption Coefficients (K) ranging from 1.1 for acid-washed sand to over 300 for one soil. An inverse relationship between tracer size and K was observed with six of the eight soils, indicating that smaller fragments are sorbed preferentially versus larger fragments in these soils; No significant correlation between K and the organic carbon contents, clay contents, pHs, or cation exchange capacities of the model soils was observed.

引用

页码：393 / 396

页数：4

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