OPTIMIZING COUPLED ENZYME ASSAYS

被引:55
作者
CLELAND, WW
机构
[1] Department of Biochemistry, University of Wisconsin, Madison
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
D O I
10.1016/0003-2697(79)90055-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Equations are given for minimizing the cost and calculating the maximum practical rates for coupled assays when two or more coupling enzymes are involved, and a lag of a predetermined size can be tolerated. The lag caused by obligatory mutarotation of an intermediate is also calculated. © 1979.
引用
收藏
页码:142 / 145
页数:4
相关论文
共 3 条
  • [1] COUPLED ENZYME ASSAYS - GENERAL EXPRESSION FOR TRANSIENT
    EASTERBY, JS
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 293 (02) : 552 - 558
  • [2] REACTION OF HEXOKINASE WITH EQUILIBRATED D-GLUCOSE
    WURSTER, B
    HESS, B
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1973, 36 (01): : 68 - 71
  • [3] Wurster B, 1975, Methods Enzymol, V41, P488